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Called "Prok" as an adult (short for Professor Kinsey), Alfred Kinsey, Jr. (Liam Neeson) has been interested in biology since he was a child growing up in the early twentieth century, despite the criticisms of such being evil nonsense from his overbearing and devoutly Christian father, Professor Alfred Seguine Kinsey (John Lithgow). Prok goes on to become a biology professor at Indiana University, initially focusing on the study of gall wasps. But those studies, in combination with questions from his students, coming to terms with the needs of sex with his own wife, a former student of his named Clara McMillen (Laura Linney) (whom he calls Mac), and what he sees as the gross misinformation on the subject currently within popular belief makes him change his focus to human sexuality. Many of those gross untruths - as he sees them - are that oral sex and masturbation cause a slew of maladies, which are perpetuated by what is presented in the university's hygiene class taught by Professor Thurman Rice (Tim Curry). With the approval of faculty head Herman Wells (Oliver Platt), Prok starts teaching his own wildly popular marriage course, talking about sex in a straightforwardly academic fashion. When he sees that morals don't necessarily mirror sexual behavior, he decides to conduct a massive sex survey with the assistance of students Wardell Pomeroy (Chris O'Donnell), Clyde Martin (Peter Sarsgaard), and Paul Gebhard (Timothy Hutton). He faces the issue of the funding required for the survey and public and political response to this controversial, and oft seen as taboo, subject. But in conducting the survey, he and Mac also face the issue of their own sexuality in relationship to their marriage, and the emotional and cultural aspects behind it, such as the notion of love.