Martyrs (1999)

When Isaac and Samuel return to France for their brother Solomon's funeral, a dark secret is revealed. Isaac's wife Alethia, a vampire that he was supposed to have killed centuries earlier, is still alive. She's captured a holy relic and is bartering it for Isaac's life. A new member of the church is recruited to assist in the controversial new development. The battle of good and evil is complicated by dark family secrets that could bring an end to the order of the vampire hunters.